earticle

영어

In this study, we optimized the important process parameters of crystallization to obtain a high purity and yield of glycopeptide antibiotics vancomycin in a purification step. The optimal ratio of acetone/water, pH, conductivity, and initial vancomycin concentration were 3.5/1 (v/v), 2.5, 20 (ms/cm), and 0.1 (g/mL), respectively. The morphology of the vancomycin crystal was analyzed during crystallization using a video microscopy system. The vancomycin was efficiently purified by cryatallization, increasing in purity from 88% to over 97%, with a yield of 95%.