earticle

영어

Microalgae are widely used as live feeds, cosmetics, human food supplements, biomedicine and etc. Especially, the use of microalgae for a potential renewable liquid fuel source was proposed by many reports. In this study, characteristics of five microalgae: Tetraselmis suecica, Phaeodactylum tricornutum, Chaeotoceros calcitrans, Isochrysis galbana and Nannochloropsis oculata were examined on the ability to store intracellular neutral lipid and growth potential. The microalgae were grown in sterilized seawater with f/2 medium nutrients and photoautotrophically in batch cultures where inorganic CO2 from the atmosphere was the only source of carbon. Aeration was provided by air stone at regular pressure. The temperature was kept at 20℃. The strains were inoculated into the medium (18 L) in a 20-L column tank. Range of inoculation density was adjusted between 20~40ⅹ104cells/ml. A fluorescent spectrometric method was applied for lipid contents analysis. In the results, Specific growth rate of N. oculata was higher (0.325/day) and the lipid content of C. calcitrans estimated by fluorescence intensity (au /106 cells) was higher than other strains.