earticle

영어

White Spot Syndrome Virus (WSSV), an enveloped and rod-shaped virus, is one of the most virulent pathogen, causing high mortality of cultured shrimp1) and large economic loss in shrimp culturing industry. The virus particle was consists of at least five major proteins VP19, VP26, VP28, VP281 and VP466.2) This study was carried out Reverse Transcription-PCR (RT-PCR) for the identification of various WSSV infection stages. The RT-PCR is widely used for the quantification of steady-state mRNA levels and is a critical tool for molecular medicine and biotechnology. A diluted WSSV stock (1:200) was used for WSSV challenge test. At 11 days of post challenge, the shrimp in the positive control with WSSV showed 100% mortality. In this study, total RNA was obtained from frozen shrimp organs at various infection stages using RNA isolation kit (Takara, Japan). Isolated RNA was reverse-transcribed into complementary DNA (cDNA) using the reverse transcription reaction kit (Takara, Japan). The resulting cDNA was amplified using β-actin, VP19, and VP26 sets. From these results, the difference expression patterns were identified various WSSV infection stages.