earticle

영어

Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from the plant cell cultures of Taxus chinensis. Crude paclitaxel was dissolved in methanol and the solution was diluted with distilled water to 61.5% (v/v) methanol. The water feeding and mixing method of fractional precipitation were evaluated in terms of the yield, purity and precipitation time of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v) , and storage time ( 7 hr and 14 hr ). The maximum purity (~57.4%) and yield (~81.8%) of paclitaxel were obtained at pH 8 with ammonium phosphate buffer. The yield and purity of paclitaxel were increased by repeated intermittent addition of ammonium phosphate buffer during fractional precipitation. This evaluation of fractional precipitation process serves to minimize solvent usage and the size and complexity of the high performance liquid chromatography operation required for paclitaxel purification.