earticle

영어

The bacterial ghost system showed a potential as a vaccine candidate against various fish pathogens. It provides versatile carrier functions for foreign antigens with natural intrinsic immune properties. The optimization of medium for E. coli K-12/pHCE-InaN-GAPDHGhost27 were determined as 10 g/L glucose, 5 g/L yeast extract, 50 mg/L thiamine with Riesenberg's semi-defined medium. The optimal batch fermentation parameters were determined as 10% inoculum size, 300 rpm agitation and 1.5 vvm aeration for the high cell density production. The optimal fed-batch process for high cell density fermentation of E. coli K-12/pHCE-InaN-GAPDH-Ghost27 was developed. The nutrient feeding strategy with Riesenberg's defined medium during fed-batch process was evaluated. The fermentation was conducted in four phases as follow: initial batch phase, fed-batch phase, temperature induction phase and heat shock phase. The maximum bacterial ghost vaccine was obtained about 40 g dcw/L. The protective efficacy of GAPDH ghost cell vaccination was determined by challenging immunized olive flounder with live S. iniae. Therefore, the ghost E. coli/ GAPDH antigen from S. iniae could be used as an effective vaccine in aquaculture.