earticle

영어

In this research, we successfully evaluated the dual biokinetic parameters between
Nitrosomonas nitrosa and Nitrosomonas aestuarii using the new mathematical algorithm
into which Monod model was modified. The process using industrial wastewater including 300 mg/L ammonium nitrogen was operated in the batch mode. From its molecular analysis, it was identified that there were Nitrosomonas europaea, N. nitrosa and N. aestuarii in the system through 16S rRNA DGGE/PCR. Furthermore, as a result of quantitative analysis with real-time quantitative PCR, it was investigated that the number of N. europaea could be nearly negligible since its population size was one hundred times smaller than the total one of other species in overall process of ammonia oxidation. Therefore, the dual kinetic algorithm, which has already been mentioned above, was applied to only two species, N. nitrosa and N. aestuarii. The three ordinary differential equations were numerically solved by 1st and 5th order Runge-Kutta Fehlberg methods. The solution calculated by the methods was compared with the experimental data in order to calculate the total sum of squares of residuals. Only in the final place, the error was numerically minimized by Levenberg-Marquardt method which is widely used as optimization tools. The values of μmax, Ks, Y and kd of N. nitrosa were 1.63 d-1, 7.3 mg NH4 +-N/L, 6.2 × 108 copy numbers/mg NH4 +-N and 0.2 d-1 , respectively. Also, those of N. aestuarii were 1.06 d-1, 19.5 mg NH4 +-N/L, 7.8 × 104 copy numbers/mg NH4 +-N and 0.036 d-1, respectively.