earticle

영어

This experiment is concerned with optimization of culture medium by C. lytica LBH-14. The one-factor-at-a-time method was used to investigate the effect of carbon & nitrogen sources, and initial pH on production of carboxymethylcellulase (CMCase) and cellobiase by C. lytica LBH-14. Base on the result of one-factor-at-a-time experiment, L25-53 was performed to measure combined effects of carbon source & nitrogen source, and initial pH on production of CMCase and cellobiase. Optimal conditions for production of CMCase were 10.0% (w/v) rice bran, 0.75% (w/v) ammonium nitrate, and initial pH 6.00, whereas those of cellobiase were 10.0% rice bran, 0.50% ammonium nitrate, and the initial pH of 7.50. On basis of intuitive analysis and statistical calculations using data from an orthogonal experiment, ammonium nitrate was found to be the most important factor for production of the CMCase by C. lytica LBH-14, whereas the most important factor for production of cellobiase was rice bran.