earticle

영어

The mammalian cells contain lysosomes, and the lysosomal enzymes are localized intralysosomally. In response to certain signals they are trigger apoptotic cell death and degradation of bacteria. Therefore, in this study, HeLa cells were exposed by H2O2 and 6-hydroxydopamine (6-OHDA), we confirmed that fluorescent intensities and size of each lysosomes were changed as concentration of H2O2 and 6-OHDA. In addition, activity of cathepsinB, D as lysosomal enzymes in lysosomes have shown to be different each concentrations. In addition, the lysosomes isolated from HeLa cells showed antimicrobial activity to Escherichia coli that positively correlated with the pH of the phosphate buffer as a dissolving solvent. As a results of the lysosomes isolated from H2O2 and 6- OHDA treated HeLa cells showed higher antimicrobial activity than those isolated from normal HeLa cells. We found that the lysosomes were activated by H2O2 and 6-OHDA. Moreover, activated lysosomes are enhanced antimicrobial effect. Therefore, it suggests that lysosomes isolated from HeLa cells can be used as antimicrobial agents. Acknowledgement: This study was supported by the 21C Frontier Microbial Genomics and Applications Center Program, grant No. 11-2008-10-002-00, Ministry of Education, Science & Technology, Republic of Korea. The authors are grateful for their supports.