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젊은 과학도 구두발표(한국어발표)

Optimization of rhBMP-7 Production by Bacillus subtilis using Response Surface Methodology

원문정보

Chun-Kwang KIM, Jong Il RHEE

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.105-105
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Bone morphogenetic protein-7 (BMP-7) is a member of the BMP subfamily of the transforming growth factor ß (TGF-ß) superfamily. It induces bone formation and thus renders it to a protein of pharmaceutical importance. In this work, response surface methodology (RSM) was employed to optimize the cultivation of Bacillus subtilis pBPT62 for the enhancement of rhBMP-7 production. A fractional factorial design was applied to study the main factors that affect the rhBMP-7 production. A few such as incubation time, starch concentration and lactose concentration were identified as most significant for high production of rhBMP-7 and the central composite design were adopted to derive a statistical model with these variables. In addition, the time course of rhBMP-7 production was also described under the optimized medium composition.

키워드

저자정보

  • Chun-Kwang KIM Dept. of Material and Biochemical Engineering, Research Center for Biophotonics, Center for Functional Nano Fine Chemicals, Chonnam National University, Gwangju, 500-757.
  • Jong Il RHEE School of Applied Chemical Engineering, Research Center for Biophotonics, Center for Functional Nano Fine Chemicals, Chonnam National University, Gwangju, 500-757.

참고문헌

자료제공 : 네이버학술정보

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