earticle

영어

Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is known as a simple and rapid molecular marker detection technique due to its high sensitivity and great reproducibility with conformation dependent separation of single strand DNA.However, conventional linear polyacrylamide (LPA) blending polymer matrix generally used for CE-based analysis has poor resolution in CESSCP analysis since it is optimized for molecular weight dependent separation. Therefore, new polymer matrix suitable for CE-SSCP analysis is required. In this study, we checked a number of polymers for CESSCP analysis based on its resolving potential using 255bp DNA fragments in variable region of 16S rRNA gene of Yersinia enterocolitica, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae. Among three polymers tested in this study, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer (PEO-PPO-PEO) showed the high resolution. After carrying out further optimization including molecular weight, PEO composition, and concentrations, high resolving CE-SSCP analysis system could be obtained. The results also displayed a strong potentials in many applications in CE-SSCP.