원문정보
초록
영어
The Nexgen is focusing on development of plant molecular farming based diagnostic kits which could be fast marketable. These kinds of products may have advantages over injectible pharmaceuticals as follow: 1) part of pharmaceutical industries, 2) less regulatory affairs,
3) fast market penetration, 4) shortens investment pregnancy period, 5) requires tiny amount of proteins, 6) requires less facilities, 7) removes false positive reaction caused by E.coli produced antigenic proteins, 8) applicable to diagnostic system for epidemic and autoimmune diseases, 9) over $30B world market Plantized(amino acid sequences as original but optimized DNA sequences with plant codon usage and removing of intron like sequences in plant) human Thyroid Stimulating Hormonal Receptor(TSHR) gene and nucleocapsid genes of Epidemic
Hemorrhagic Fever viruses such as Hantaan, Puumala and SinNombre were designed and synthesized then cloned into marker free constructs. Proteins of interest were successfully expressed in plant by Agrobacterium -mediated transient expression system which was
suitable for small scale production in the lab to avoid neither environmental nor regulatory matters. Plant-made antigenic proteins were used to prepare ELISA and Rapid Strip kits that are currently being reviewed by KFDA and USFDA. Transgenic plants using oriental melon and cucumber are being produced in case of further mass production of antigenic proteins in the lab then some parts of transgenic tissues will be relocated into the enclosed vessel system for
organ culture in the surely secured in-house facility. There would not be open field trials for transgenic plants to avoid public concerns.
