earticle

영어

By immunizing rabbits with HGF/cMet complex, we successfully generated a monoclonal antibody that inhibits HGF/cMet interaction, and blocks the biological function mediated by HGF in vitro and in vivo. To define epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF/cMet interaction. No humoral response was induced to
this epitope-mimicking peptide when immunization was done with HGF alone. This rabbit monoclonal antibody was successfully humanized, optimized for process development and superior in vivo characteristics and now under active characterization.