원문정보
초록
영어
Synthetic biology is an emerging field that aims to design and build synthetic networks of biological reactions using well-characterized biomolecular components and genetic modules. While the current efforts in synthetic biology rely mostly on the engineering of living host cells, progress in the in vitro synthesis of DNA, RNA or proteins make it possible to construct truly 'synthetic' networks of biological functions. In particular, recent advances in the techniques of cell-free protein synthesis are expected to provide a versatile platform for the in
situ construction of in vitro networks of important proteins. Compared to in vivo synthetic biology, in vitro synthetic biology offers substantial advantages including increased flexibility of engineering and less background interference with the synthetic enzyme network.
Herewith, we present and discuss our recent achievements in the development of cell-free protein synthesis systems, which have been modified in various configurations to allow the flexible manipulation of gene expression and reaction conditions. We expect that the
developed systems will be used for studying focused and intentional regulation of protein synthesis and other biological functions in the context of synthetic biology.