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영어

The Designer’s Synthetic Biology Cluster is dedicated to provide total synthetic biology solutions -
from Gene Design to Directed Protein Evolution & Expression, which is sponsored and supported by DDI (Daedeok Innopolis) and MKE (Ministry of Knowledge Economy) of Korea. This program is aimed at establishing a foundation for artificial synthetic technologies from genes to proteins by
integrating all the steps, from the design of a functional new gene to the high speed synthesis of the gene to the directed evolution of the protein and gene and its expression. It also aims to synthesize the total genome and develop the functionally improved proteins that are needed in the White, Red, and Green Bio fields. The Designer’s Synthetic Biology Cluster, which will lead the era of Bio 2.0, the nextgeneration bio-industry era, will be created and be expanded into enterprises and research institutes that need the synthetic biology-based technology developed by this project. In this presentation, we will report our works – Gene Design Software, HT Gene Synthesizer and Kit, Mega-base Oligo Synthesizer- ranging from the design of a functional new gene to the high speed synthesis of the gene. One of the most important platform technologies in implementing Synthetic Biology is the software used to design the synthetic gene sequence necessary for the gene or set of genes that the customer wants. We firstly developed, in collaboration with KAIST, this software, which is composed of codon optimization, mRNA secondary structure estimation and oligonucleotide design. This software enables the design of genes by optimizing the codon specific for the host and different factors related to protein
expression so that the expression of the protein, which is the final target of gene synthesis, can be implemented. The designed genes are automatically and high-Throughput synthesized on the HT Gene Synthesizer, on the basis of Accuracy Position Control Technology and Micro Sealing Technology, and the Peltier method. The gene synthesis kit, a ready-to-use format including all components for gene synthesis was also developed. Sub-nanomole Scale Oligo Synthesizer is under development, having the capability to synthesize with sub nano mole at an initial synthesis scale in a rapid and economical cycle time. It ensures the quality and purity of the synthesized oligonucleotides and is optimized to synthesize oligos for gene synthesis, gene libraries and codon bias adjustments. On this platform, we will discuss in detail during the presentation.