earticle

영어

Naringenin is a bioactive flavanone containing antioxidative, anti-inflammatory, and anticarcinogenic properties.The inhibitory effects on hyaluronidase of naringenin and its novel derivatives were evaluated. Among these flavonoids at 200µM concentration, 7-O-butyl naringenin had the highest inhibitory effect on hyaluronidase with 44.84%. In addition, Fornaringenin at concentrations of 0, 150, and 190µM, the apparent Michaelis constants (appKm) were calculated to be 0.60±0.02,0.43±0.02, and 0.41±0.01mg/mL of substrate, respectively; for 7-O-butyl naringenin at 0, 20, and 30µM concentrations,those were 0.44±0.03 and 0.27±0.03 mg/mL, respectively. The Vmax values at 150 and 190µM naringenin were 0.59±0.02and 0.56±0.01 mg/mL/min, respectively; and those at 20 and 30µM 7-O-butyl naringenin were 0.50±0.02 and 0.33±0.02mg/mL/min, respectively. However, the slopes of each inhibitory reaction were not significantly different. Therefore,naringenin and 7-O-butyl naringenin were shown to be uncompetitive inhibitors. These results demonstrate the potential use of7-O-butyl naringenin as an anti-inflammatory substance.