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This study evaluated glucose effect on Listeria monocytogenes survival under gamma irradiation and NaCl stress.L. monocytogenes in phosphate buffered saline (PBS) plus glucose (0-4%) was treated with gamma irradiation (0-0.5 kGy),and the samples were then exposed to NaCl (0-9%) in tryptic soy agar plus 0.6% yeast extract. D10 and t3D values weredetermined, and a model for prediction of D10 values was developed. Cell counts of L. monocytogenes reduced as irradiationdose increased, and L. monocytogenes in PBS (no glucose) was more sensitive to irradiation and NaCl compared to those inPBS (2 or 4% glucose). D10 values were 0.07-0.1, 0.12-0.16, and 0.13-0.15 kGy for 0, 2, and 4% glucose, respectively. The t3Dvalues were 0.22-0.3 (0% glucose), 0.35-0.48 (2% glucose), and 0.40-0.44 (4% glucose). A model performance wasacceptable. These results indicate that glucose in foods would increase the resistance of L. monocytogenes to gammairradiation and NaCl stress.