earticle

영어

The present study was conducted to determine the protective ability of the ethanol extracts of Hypericumscabroides Robson & Poulter (HS) and Hypericum triquetrifolium Turra (HT) against the protein oxidation and DNA damageinduced by Fenton system. The ability of HS and HT to prevent oxidative damage to bovine serum albumin (BSA) induced byFe3+/H2O2 and ascorbic acid was investigated. The ethanol extracts of HS and HT at different concentrations (50-1,000µg/mL) efficiently prevented protein oxidation induced by hydroxy radical as assayed by protein oxidation markers includingprotein carbonyl formation (PCO) and polyacrylamide gel electrophoresis. The effect of ethanol extracts of HS and HT onDNA cleavage induced by UV-photholysis of H2O2 using pBluescript M13+ plasmid DNA were investigated. These extractssignificantly inhibited DNA damage induced by reactive oxygen species (ROS). Therefore, HS and HT extracts may be usefulin the food industry as effective synthetic antioxidants.