earticle

영어

A method was developed using enhanced liquid chromatography coupled with electrospray ionization massspectrometry for the analysis and quantitation of 2 main potato glycoalkaloids, α-chaconine, and α-solanine, without any pre-concentration or derivatisation steps. Calibration curves generated by this technique exhibited a linear dynamic range from0.025 to 50µg/mL and from 0.05 to 50µg/mL for α-chaconine and α-solanine, respectively. Matrix effects were evaluated bycomparing calibration curves measured in matrix-matched and solvent-based systems. Ion suppression due to matrix effectswas weak and extraction recoveries of 88 to 114% were obtained in different sample matrices spiked with analyteconcentrations ranging from 15 to 35µg/mL. Potatoes that had been genetically modified to tolerate glufosinate contained thesame glycoalkaloid levels as their non-transgenic counterpart. We suggest complementing compositional comparisonassessment strategy by validating quantitative analytical methods for the toxic glycoalkaloids in potato plants.