earticle

영어

The aerial parts of garland (Chrysanthemum coronarium L.) were extracted in 80% aqueous methanol (MeOH)and the concentrated extract was then partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O, successively.EtOAc and n-BuOH fractions resulted in 4 glycerides with the application of octadecyl silica gel and silica gel columnchromatography. The chemical structures of the glycerides were determined using several spectroscopic methods, includingnuclear magnetic resonance (NMR) and mass spectrometry (MS) as (2S)-1-O-palmitoyl-sn-glycerol (1), (2S)-1-O-oleoyl-2-O-oleoyl-3-O-β-D-galactopyranosyl-sn-glycerol (2), (2S)-1-O-palmitoyl-2-O-linoleoyl-3-O-phosphorouscholine-sn-glycerol (3),and (2S)-1-O-linolenoyl-2-O-palmitoyl-3-O-[α-D-galactopyrasyl-(1→6)-β-D-galactopyranosyl]-sn-glycerol (4). The free fattyacids of these glycerides were determined with gas chromatography (GC)-MS analysis following alkaline hydrolysis andmethylation. These glycerides demonstrated an inhibitory effect on acyl-CoA: cholesterol acyltransferase (ACAT, compound1: 45.6±0.2% at 100µg/mL), diacylglycerol acyltransferase (DGAT, compound 1: 59.1±0.1% at 25µg/mL), farnesyl proteintransferase (FPTase, compound 2: 98.0±0.1%; compound 3: 55.2±0.1% at 100µg/mL), and β-secretase (IC50, compound 4:2.6µg/mL) activity. This paper is the first report on the isolation of these glycerides from garland and their inhibitory activityon ACAT, DGAT, FPTase, and β-secretase.