earticle

영어

Frequent outbreaks of foodborne illness have been increasing the awareness of food safety. Conventional methodsfor pathogen detection and identification are labor-intensive and take days to complete. Some immunological, rapid assays aredeveloped, but these assays still require prolonged enrichment steps. Recently developed biosensors have shown potential forthe rapid detection of foodborne pathogens. In this study, an impedimetric biosensor was developed for rapid detection ofSalmonella entritidis in food sample. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by usinga semiconductor fabrication process. Anti-Salmonella antibodies were immobilized based on neutravidin-biotin binding on thesurface of the IME to form an active sensing layer. To evaluate the effect of electrode gap on sensitivity of the sensor, 3 typesof sensors with different electrode gap sizes (2, 5, and 10µm) were fabricated and tested. The impedimetric biosensor coulddetect 103 CFU/mL of Salmonella in pork meat extract with an incubation time of 5 min. This method may provide a simple,rapid, and sensitive method to detect foodborne pathogens.