earticle

영어

Near infrared reflectance spectroscopy (NIRS) was used as a rapid and non-destructive method to determine theprotein content in intact and ground seeds of pea (Pisum sativum L.) germplasms grown in Korea. A total of 115 sampleswerescanned in the reflectance mode of a scanning monochromator at intact seed and flour condition, and the reference values forthe protein content was measured by auto-Kjeldahl system. In the developed ground and intact NIRS equations for analysis ofprotein, the most accurate equation were obtained at 2, 8, 6, 1 math treatment conditions with standard normal variate anddetrend scatter correction method and entire spectrum (400-2,500nm) by using modified partial least squares regression(n=78). External validation (n=34) of these NIRS equations showed significant correlation between reference values andNIRS estimated values based on the standard error of prediction (SEP), R2, and the ratio of standard deviation of referencedata to SEP. Therefore, these ground and intact NIRS equations can be applicable and reliable for determination of proteincontent in pea seeds, and non-destructive NIRS method could be used as a mass analysis technique for selection of highprotein pea in breeding program and for quality control in food industry.