원문정보
Spore Production of Entomopathogenic Fungus, Beauveria bassiana 726, Using Molasses
초록
영어
To optimize the culture conditions for Beauveria bassiana 726, the effects of culture medium, pH, and temperature on mycelium and spore production were investigated. The optimum temperature and pH for the cultivation of B. bassiana 726 were 28 ℃ and 5.0, respectively. The optimized medium was composed of 1.0~2.0% total sugar from molasses, 0.5% corn steep liquor and 0.05% KH2PO4. In the cultivation of B. bassiana 726 with the optimum medium, the specific growth rate and substrate utilization were well-fitted with the proposed kinetic model in the shake flask and stirred tank reactor. When the fed-batch cultivation using carbon suorce, nitrogen source, and mineral salt as a feeding medium was compared with batch cultivation in stirred tank reactor, mycelium (12.7 g/L) and spore production (5.4x108/mL) were enhanced up to 110% and 85%, respectively.
목차
재료 및 방법
사용균주 및 배지
배양방법
분석방법
결과 및 고찰
배양온도와 초기 pH의 영향
당일 농도의 영향
질소원의 영향
무기염류의 영향
최적화된 생산배지에서의 포자생산
요약
감사
참고문헌