earticle

영어

Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of OD600=0.5 to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.

한국어

미나리의 엽조직을 agroinfiltration 및 동시배양을 수행하기 전에 NaOH 용액에서 3 min 처리를 실시하였다. MTT를 이용한 세포사멸실험에서 0.7% NaOH 용액처리까지는 엽조직 세포활성에 안전한 것으로 판단되었다. GUS 효소활성의 형광분석 결과 0.5% NaOH를 처리한 미나리 엽조직에 대하여 Agrobacterium cells (OD600=0.5에서 1.0)을 이용한 vacuum infiltration (20 min)을 실시할 경우 효율적 형질전환이 이루어짐을 알 수 있었다. 이러한 조건은 western blotting과 ELISA에 의한 HBsAg의 발현 검정에서 확인할 수 있었다.