원문정보
Betaine Production by Two Stage Culture and Elicitor in the Cell Cultures of Lycium chinense Mill
초록
영어
The effects of carbohydrates, hormones and elicitors on both cell growth and betaine production were investigated in the cell cultures of Lycium chinense Mill. The maximum effect of glucose and sucrose was observed in cells cultured in the presence of 3% and 7% for cell growth and betaine production, respectively. The effect of hormones on cell growth and betaine production was prominent in the presence of 10 M 2, 4-D, 10 M NAA and 2.5 M IAA, whereas cell growth and betaine production were excellent at 2.5 M BA and 10 M BA, respectively. Abiotic elicitors such as KCI, MnCl2 and NaCl exhibited an inhibitory role on cell growth in all treatment groups. Betaine production was increased according to increase of concentration of abiotic elicitors. methanol-soluble and insoluble components as biotic elicitor remarkably inhibited cell growth from 2 mg and 6 mg, respectively. Betaine production was increased maximally at 2 mg of biotic elicitors. When growth medium was switched to production medium at two stage culture, it resulted that cell fresh weight and dry weight decreased but betaine content increased about 2.2-fold.
목차
재료 및 방법
캘러스의 유기 및 배양
Betaine의 추출과 HPLC 분석
세포성장과 betaine의 생산에 미치는 당의 영향
세포성장과 betaine의 생산에 미치는 hormone의 영향
세포생장과 betaine의 생산에 미치는 abictic elicitor의 영향
배양시기별 세포생장과 betaine 생산에 미치는 bioticelicitor의 영향
세포생장과 betaine의 생산에 미치는 biotic elicitor의 영향
이단계 배양에 의한 betaine 생산
결과
세포생장과 betaine 생산에 미치는 당의 영향
세포생장과 betaine 생산에 미치는 hormone의 영향
세포생장과 betaine 생산에 미치는 abiotic elicitor의 영향
배양시기별 세포생장과 betaine 생산에 미치는 biotic elicilor의 영향
세포생장과 betaine 생산에 미치는 biotic elicitor의 영향
이단계 배양의 영향
고찰
요약
참고문헌