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논문검색

Pseudomonas putida F1과 Burkholderia cepacia G4에 의한 BTEX, trichloroethylene 분해

원문정보

Degradation of BTEX and Trichloroethylene by Pseudomonas putida F1 and Burkholderia cepacia G4

이승우, 이준명, 장덕진

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Two cometabolic trichloroethylene (TC) degraders, Pseudomonas putida F1 and Burkholderia (Pseudomonas) cepacia G4, were found to catabolize phenol, benzene, toluene, and ethylbenzene as carbon and energy sources. Resting cells of P. putida F1 and B. cepacia G4 grown in the presence of toluene and phenol, respectively, were able to degrade not only benzene, toluene and ethylenzene but also TCE and p-xylene. However, these two strains grown in the absence of toluene or phenol did not degrade TCE and p-xylene. Therefore, it was tentatively concluded that cometabolic degradation of TC and p-xylene was mediated by toluene dioxygenase (P. putida F1) or toluene-2-monooxygenase (B. cepacia G4). Maximal degradation rates of BTEX and TCE by toluene- and phenol-induced resting cells of P. putida F1 and B. cepacia G4 were appeared to be 4-530 nmol/(minmg cell protein) when a single compound was solely served as a target substrate. In case of double substrates, the benzene degradation rate by P. putida F1 in the presence of toluene was decreased up to one seventh of that for the single substrate. TCE degradation rate was also linearly decreased as toluene concentration increased. On the other hand, toluene degradation rate was enhanced by benzene and TCE. For B. cepacia G4, degradation rates of TCE and toluene increased 4 times in the presence of 50 M phenol. From these results, it was concluded that a degradation rate of a compound in the presence of another cosubstrate(s) could not be predicted by simply generalizing antagonistic or synergistic interactions between substrates.

목차

서론
 재료 및 방법
  균주 및 배양방법
  탄소/에너지원 검색
  휴지세포(resting cell)의 제조
  분해실험
  액상내 물질 농도 계산
  분해속도의 계산
 결과 및 고찰
  Toluene oxygenase 발현 유도
  휴지세포에 의한 BTEX,  TEC 분해
  두 가지 물질 공존시 분해속도
 요약
 감사
 참고문헌

저자정보

  • 이승우 Seungwoo Lee. 명지대학교 화학공학과
  • 이준명 Junmyoung Lee. 명지대학교 화학공학과
  • 장덕진 Deokjin Jahng. 명지대학교 화학공학과

참고문헌

자료제공 : 네이버학술정보

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