원문정보
Purification and Characterization of Recombinant Monellin Produced from Yeast Culture Medium
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초록
영어
The monellin, a sweet-taste protein, was expressed and secreted in Saccharomyces cerevisiae. The secreted menellin was concentrated using an ultrafiltration membrane with a nominal molecular weight cut off of 3,000 or by ammonium sulfate precipitation. The monellin was purified by G-25 gel filtration chromatography, followed by CM-Sepharose ion exchange chromatography. The purified monellin was characterized by SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis) and PHLC. The molecular weight of monellin was found to be 10,700 dalton, and its purity was over 95%.
목차
서론
재료 및 방법
제조합 효모
발효
발효액 처리
크로마토 그래피
분석방법
결과 및 고찰
발효
G-25 크로마토그래피
CM-Sepharose 크로마토그래피
멱삼, 이온교환 HPLC와 젤 여과 HPLC
요약
참고문헌
재료 및 방법
제조합 효모
발효
발효액 처리
크로마토 그래피
분석방법
결과 및 고찰
발효
G-25 크로마토그래피
CM-Sepharose 크로마토그래피
멱삼, 이온교환 HPLC와 젤 여과 HPLC
요약
참고문헌
저자정보
참고문헌
자료제공 : 네이버학술정보