원문정보
Production of Agarooligosaccharides using of Agarase from marine Bacterium Bacillus cereus ASK202
초록
영어
An agarase was partially purified from the culture broth of marine bacterium Bacillus cereus ASK202. Optimal pH and temperature of this agarase were found to be 7.0 and 40℃, respectively. The maximum productivity of agarooligosaccharides was obtained from 0.3 %(w/v) agar by using of 1 unit agarase. As the results of TLC and HPLC analysis, these oilgosaccharides consisted of neoagarobiose, neoagarotetraose and neoagarohexaose. Under the optimal reaction conditions, 77.5 %(w/v) neoagarobiose and 6.2 %(w/v) neoagarotetraose were produced from agar and the conversion yield of total agarooligosaccharides was 83.7 %(w/v) after for 2 h reaction at 40℃.
목차
재료 및 방법
시약
미생물 배양 및 배지조성
조효소의 제조
환원당 및 전당량의 측정
분해율(전환율) 측정
Thin layer chromalography(TLC)
생성된 올리고당의 분석
결과 및 고찰
한천분해효소의 활성에 미치는 온도 및 pH의 영향
한천올리고당의 생성에 미치는 초기기질 및 효소농도의 영향
생성 올리고당의 분석
최적반응조건하에서 한천올리고당의 생산
요약
사사
참고문헌
