원문정보
Toxicity Evaluation of Hazardous Contaminants by Measuring Lag Periods and Specific Growth Rates of a Test Microorganism
초록
영어
Among 31 water-born microbial strains isolated from various sites in Korea, strain DJ-4 was selected as a test organism for toxicity measurements in that its growth was completely inhibited by the presence of 668.4 mg/L of chloroform and 297.5 mg/L of toluene in the liquid LB medium whereas others did not. It was observed that lag periods and specific growth rates of DJ-4 batch vial cultures were prolonged and decreased, respectively, by phenol, benzene, toluene, ethylbenzene, p-xylene, perchloroethylene, trichloroethylene, and chloroform at the concentrations between 3.6 and 417.8 mg/L. There changes were found to be linear with respect to the concentrations of the toxic compounds. From the first-order regression equations, 50% effective concentrations (EC50 for concentrations of toxic compounds causing 50% decrease of specific growth rates and EC50lag for 50% increase of length of lag periods) were calculated for each compounds. By comparing DJ-4 EC50 values with Daphnia LC50's from a literature for benzene, ethylbenzene, toluene, and trichloroethlyene, it was concluded that microbial specific growth could be a new, fast, and reliable parameter for toxicity tests.
목차
재료 및 방법
균주 및 배양방법
지표균주 선정
액상내 독성물질의 농도 계산
DJ-4 성장곡선의 작성
50% 유효농도(EC50)
결과 및 고찰
지표균주의 선정
독성물질 존재시 DJ-4의 성장거동
독성물질의 농도가 지연기에 미치는 영향
독성물질에 의한 비성장속도의 감소
EC501ag와 EC50u의 관계
EC50u와 Daphnia LC50의 상관관계
요약
감사
참고문헌