원문정보
Production of Red Pigment by Serratia sp. KH-95 and its Cultural Properties
초록
영어
Optimal media and cultural conditions for the production of prodigiosin-like pigment were established using Serratia sp. KH-95. Glucose and phosphate(K2PO4) stimulated the cell growth, but inhibited the production of pigment at concentration levels of above 10 g/L and 2.0 g/L, respectively. Addition of soy been oil or rice oil to the production medium accelerated cell growth up to more than 2-3 times, but the production of prodigiosin increased about 15-20% in spite of the good cell growth. The effect of pH on the production of pigment was investigated in a 5 liter-bioreactor. When the pH of culture broth was maintained below 8.0, most of pigment was attached to the surface of cells. When the pH of culture broth was above 8.5, however, about 70% of total pigment was suspended in the supernatant of the broth. The cell growth and production of pigment were inhibited at dissolved oxygen concentration of below 10% of air-saturation.
목차
재료 및 방법
사용균주
배지조성 및 배양방법
균체농도의 측정방법
적색색소의 측정방법
결과 및 고찰
포도당 및 인산이 균체의 성장 및 색소 생산에 미치는 영향
대두유 및 미강유가 색소 생성에 미치는 영향
미량원소(Fe-+, Zn++. Mn++ Cu++)들이 색소 생산애 미치는 영향
생물반응기에서 pH가 균체의 성장 및 색소 생성에 미치는 영향
생물반응기에서 용존산소 농도가 균체의 성장 및 색소 생산에 미치는 영향
요약
참고문헌