원문정보
Modelling of a Biomolecular Processing for the Production and Secretion of Monoclounal Antibody
초록
영어
To analyze the unique aspects of biomolecular processing for monoclonal antibody (MAb) production and secretion, the simple working model based on 3-compartment (endoplasmic reticulum, Golgi apparatus, and extracellular medium) was developed. Based on in vitro MAb assembly experimental results, the kinetic model for MAb assembly in the endoplasmic reticulumn was proposed. The dynamics of MAb assembly and secretion was simulated using methematica program. According to the simulation results, the proposed 3-compartment model provides an efficient means to predict the specific MAb productivity as well as intracompartmental concentrations of MAb in endoplasmic reticulum, Golgi apparatus, and extracellular compartment model. In vivo profiles of MAb intermediates gave good agreements with the simulation profiles predicted by the intracellular compartment model. Furthermore, results of such analysis can help in directing the control strategy for optimum biomolecular processing in a mammalian cell culture system.
목차
이론
단일클론항체의 조립 반응
단일클론항체 분비공정에 대한 3 격실(Compartment) 모델
재료 및 방법
세포주 및 배양 조건
In vivo pulso labeling 실험
결과 및 고찰
단일클론항체 조립중간체의 in vivo 분석
단일클론항체 조립 및 분비공정에 대한 해석
요약
감사
용어설명
참고문헌