원문정보
The Optimum Conditions for the Production of Exo-polymer from Submerged Mycelial Culture of Ganoderma lucidum WK-003 and It's Hepatoprotective Effect
초록
영어
The optimum conditions of the production of exo-polymer by Ganoderma lucidum WK-003 and it's hepatoprotective effect was studied. Optimum conditions for the production of exo-polymer (3.18 g/l) by using shaken flask culture of G. lucidum WK-003 were pH 4.5, 30℃, 120 rpm for 18 days cultivation. Also exo-polymer productio (7.15 g/l) was optimized by 5l jar fermenter cultivation with condition of pH 4.5, 30℃, 200 rpm, 1.0 vvm for 6 days cultivation. Glutamic pyruvic transaminase(GPT) activities in serum of intoxicated Sprague-Dawley rat were decreased from 704 IU/L to 330 IU/L by oral administration of the exo-polymer (20mg/kgday)f or 4 consecutive days.
목차
재료 및 방법
사용 균주 및 배지
접종원
Polymer의 회수
pH 및 온도 조건에 따른 균사체 생장 및 Exo-ploymer 생산
교반 속도 및 배양기간에 따른 균사체 생장 및 Exo-polymer 생산
Exo-polymer 생산을 위한 발효조 배양
간 보호 효과
결과 및 고찰
배양 조건 확립
진탕 배양기간에 따른 균사체 수율 및 exo-polymer 생산
다당생산 배지를 사용한 5ℓ 발효조 배양
간 보호 효과
요약
감사
참고문헌