원문정보
Cell separation from high density culture broths of Alcaligenes eutrophus by using Al-based coagulants
초록
영어
Cell recovery from high cell density broths of Alcaligenes eutrophus by pretreatment with aluminum-based coagulants such as aluminum sulfate, polyaluminum hydrooxide chloride silicate (PACS), and polyaluminum hydrooxide chloride (Hi-PAX) was carried out. Cells coagulated with coagulants could be successfully recovered above 95-99% by centrifugation or filtration. The optimum initial pH of fermentation broths for cell recovery was in the range of 10 to 12. Optimum coagulants dosage for cell recovery increased with increasing of cell concentrations (21-160 g/L). The optimum coagulant dosages to recover cells with more than 95% cell recovery by centrifugation for the cell concentrations ranged 21-160 g/L were as follows: aluminum sulfate, 416-1708 mg Al/L; PACS, 211-826 mg Al/L; Hi-PAX, 320-960 mg Al/L. At optimum conditions for the coagulation of cells, centrifugal forces for 95% of cell recovery were dependent on the cell concentration. The centrifugal forces at 82 g/L and 160 g/L of cell concentration were only 45g and 1600g, respectively.
목차
재료 및 방법
A. eutrophus의 배양
사용한 응집체
응집체에 의한 균체 회수
결과 및 고찰
균체 회수율에 미치는 배양액의 초기 pH의 영향
균체 회수율에 미치는 응집제 첨가량의 영향
균체농도와 원심력간의 관계
최적 응집제 첨가량
요약
감사
참고문헌