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논문검색

Alcaligens eutrohus 고농도 배양액으로부터 알루미늄(Al)계 응집제를 이용한 세포분리

원문정보

Cell separation from high density culture broths of Alcaligenes eutrophus by using Al-based coagulants

조경숙, 류희욱, 정현우, 곽종운, 장용근

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Cell recovery from high cell density broths of Alcaligenes eutrophus by pretreatment with aluminum-based coagulants such as aluminum sulfate, polyaluminum hydrooxide chloride silicate (PACS), and polyaluminum hydrooxide chloride (Hi-PAX) was carried out. Cells coagulated with coagulants could be successfully recovered above 95-99% by centrifugation or filtration. The optimum initial pH of fermentation broths for cell recovery was in the range of 10 to 12. Optimum coagulants dosage for cell recovery increased with increasing of cell concentrations (21-160 g/L). The optimum coagulant dosages to recover cells with more than 95% cell recovery by centrifugation for the cell concentrations ranged 21-160 g/L were as follows: aluminum sulfate, 416-1708 mg Al/L; PACS, 211-826 mg Al/L; Hi-PAX, 320-960 mg Al/L. At optimum conditions for the coagulation of cells, centrifugal forces for 95% of cell recovery were dependent on the cell concentration. The centrifugal forces at 82 g/L and 160 g/L of cell concentration were only 45g and 1600g, respectively.

목차

서론
 재료 및 방법
  A. eutrophus의 배양
  사용한 응집체
  응집체에 의한 균체 회수
 결과 및 고찰
  균체 회수율에 미치는 배양액의 초기 pH의 영향
  균체 회수율에 미치는 응집제 첨가량의 영향
  균체농도와 원심력간의 관계
  최적 응집제 첨가량
 요약
 감사
 참고문헌

저자정보

  • 조경숙 Kyung-Suk Cho. 이화여자대학교 환경공학과
  • 류희욱 Hee Wook Ryu. 숭실대학교 환경·화학공학과
  • 정현우 Hyon Wu Chung. 숭실대학교 환경·화학공학과
  • 곽종운 Jong Woon Kwak. 경기화학공업(주) 수처리연구실
  • 장용근 Yong Keun Chang. 한국과학기술원 화학공학과 및 생물공정연구센터

참고문헌

자료제공 : 네이버학술정보

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