원문정보
Bioreactor Operating Strategy in Scultellaria baicalensis G. Plant Cell Culture for the Production of Flavone Glycosides
초록
영어
Optimal feeding strategies in bioreactor operation of Scutellaria baicalensis G. plant cell culture were investigated to maximize the production of flavone glycosides by using a structured kinetic model which can predict culture growth and flavone glycosides synthesis in a rigorous, quantitative manner. For the production of baicalin and wogonin-7-0-GA, the strategies for glucose feeding into Scutellaria baicalensis G. plant cell culture were proposed based on the model, which are a periodic fed-batch operation with maintenance of cell viability and of specific production rate respectively, and a perfusion operation with maintenance of specific production rate for baicalin and wogonin-7-0-GA. Simulation results showed that the highest volumetric concentration of flavone glycosides was obtained in a periodic fed-batch operation with maintenance of cell viability among all the suggested strategies. In the periodic fed-batch operations, the higher volumetric production of flavone glycosides was achieved compared with that in the perfusion operation. It can be concluded that a periodic fed-batch operation with maintenance of cell viability would be the optimal and practical operating strategy of Scutellaris baicalensis G. plant cell culture for the production of flavone glycosides.
목차
재료 및 방법
회분식 현탁 세포 배양 실험
분석방법
이론
Scutellraia baicalensis G. 식물 세포 성장 모델식
매개변수 추정
운전 전략의 수렵
결과 및 고찰
세포 생존도를 유지시키는 주기적 유가식 운전 전략의 현상 모사
Baicalin과 wogonin-7-O-GA의 비생산 속도를 유지시키는 주기적 유가식 운전 전략의 현상 모사
Baicalin과 wogonin-7-O-GA의 비생산 속도를 유지시키는 perfusion 운전 전략의 현상 모사
요약
사용기호
참고문헌