원문정보
Studies of Cyclosporin A Biosynthesis under the Conditions of Limited Dissolved Oxygen or Carbon Source in Fed-batch Culture
초록
영어
We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), Yx/s (0.49 g DCW/g fructose),Yp/s<(22.56 mg CyA/g fructose), and YTEX>$_p/x$ (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism.
목차
재료 및 방법
균주
배지 및 배양조건
분석방법
유가식 배양을 위한 발효조 실험
배양액의 용존산소 농도 측정 및 자동 조절
결과 및 고찰
탄소원 또는 용존산소의 정밀 조절을 위한 유가식별양법확립
용존산소와 탄소원 제한 조건이 CyA 생합성에 미치는 영향
요약
감사
참고문헌