원문정보
Adsorption Characteristic of Endo I and Exo II Purified from Cellulase by Trichoderma viride on Celluloses with Different Crystallinity
초록
영어
The adsorption behaviors of two major cellulase components, endo I and exo II, from Trichoderma viride were investigated using a-celluloses with different correlation crystallinity index(Cc) as substrates. The adsorption of cellulase enzyme components was significantly affected by the reaction condition and the physicochemical properties of the cellulose. The a-cellulose was hydrolyzed in the presence of cellulase for various periods. The correlation crystallinity index of a-cellulose increased with increasing the hydrolysis time. The adsorption was apparently found to obey the first-order kinetics, and the adsorption activation energy(Ea) was calculated from the adsorption rate constant(ka). The value of adsorption rate constant for endo I was larger than that of exo II. This means that endo I are adsorbed more rapidly than exo II. With the increase in correlation crystallinity index, the values of the adsorption rate constants for endo I and exo II decreased, respectively. The activation energy for the adsorption of exo II on the cellulose also was larger than that of endo I. Also adsorption activation energy of endo I and exo II increased with an increase in the crystallinity of sample cellulose.
목차
재료 및 방법
시약
효소 가수분해에 의한 셀룰로오스의 전처리
상관결정성 지수(Cc, correlation crystallinity index)
단백질의 정량
혼합효소의 분리 및 정제
Cellulase 성분효소의 흡착
결과 및 고찰
가수분해를 통한 셀룰로오스의 구조 변화
구조가 다른 셀룰로오스에 대한 성분효소의 흡착
요약
참고문헌