원문정보
Isolation and Characterization of a Phenol-Degrading Bacteria
초록
영어
Twelve bacterial strains capable of growing on phenol minimal medium were isolated from iron foundry activated sludge by enrichment culture, and amount them, one isolate which was the best in cell growth and phenol degradation was selected and identified as Acinetobacter junii POH. The optimal temperature, initial pH and phenol concentration in the above medium were 30℃, 7.5 and 1000 ppm, respectively. Cell growth of Acinetobacter junii POH dramatically increased 20 hrs cultivation-time and reached a almost stationary phsae 40 hrs cultivation-time then phenol was degraded about 98%. Cell growth was inhibited y phenol at concentrations over 1500 ppm. The isolate was resistant to several antibiotics as well as various heavy metal ions. The growth-limiting log P value of Acinetobacter junii POH on organic solvents was 2.9 in the LB medium. Therefore, it is suggested that Acinetobacter junii POH could be effectively used for the biological treatment of wastewater containing the presence of heavy metal ions and organic solvents.
목차
재료 및 방법
균주의 분리원과 사용배지
Phenol 분해 균주의 분리 및 선발
Phenol 분해에 따른 세포성장 특성
분리 균주의 동정
항생제 및 중금속에 대한 내성
유기용매에 대한 내성
Phenol 농도 분석방법
결과 및 고찰
균주의 분리 및 선발
분리 균주의 동정
항생제 및 중금속에 대한 내성
유기 용매에 대한 내성
Phenol 분해에 따른 세포성장과 pH의 변화
pH에 딱른 phenol 분해
온도어 따른 phenol 분해
Phenol 농도에 따른 세포성장과 분해
결론
감사
참고문헌
