earticle

영어

Harringtonine and homoharringtonine known as anti-cancer agents were isolated from Korean native plumyew(Cephalotaxus koreana) using column chromatography(CHCl3:MeOH=19:1, Rf=0.28). The structure of the mixture of two compounds was characterized by 1H-NMR. Comparison of our spectra of harringtonine and homoharringtonine with previously reported ones indicated that the two are identical. The contents of harringtonine and homoharringtonine in the needles, stems, and roots of Korean native plumyew were determined by high performance liquid chromatography(HPLC). The contents of both compounds varied with the site of location and the part of plant. The content of harringtonine was higher in needles and roots than in stems, whereas the content of homoharringtonlne was lower than harringtonine. Homoharringtonine contents in needles at Mt. Palgong, Mt. Dukyu, Mt. Baekyang, Mt. Jiri, and Namhae were higher than in stems and roots. But homoharringtonine contents in needles al Mt. Jokye and Jindo were lower than in stems and roots.

한국어

한국산 개비자로부터 alkaloid계통의 성분인 har­r ringtonine과 homoharringtonine의 혼합물을 col umn chromatography (CHCb: MeOH == 19: 1, Rf== 0 0.28)로 분리, 정제하였다. 이 두 물질의 혼합물의 화학 구조는 IH-NMR에 의해 결정하였으며 이미 보고된 spectra와 이 두 물질의 혼합물의 spectra를 비교하여 harringtonine과 homoharringtonine 임을 확인하였다. 한국산 개비자의 잎, 줄기, 뿌리에서의 harringtonine과 homoharringtonine의 함량은 고속 액체 크래마토그래피에 의해 결정하였다. 두 물질의 함량은 서식지, 식물의 부위에 따라 상이하였다. Harringtonine의 함량은 줄기보다 잎과 뿌리에서 높 았고, homoharringtonine의 함량은 harringtonine 함량보다 낮았다. 팔꽁산, 덕유산, 백양산, 지리산 빛 남해에서의 잎의 homoharringtonine 함량은 줄기와 뿌리에서 높게 나왔으며, 조계산과 진도에서는 잎의 함량이 뿌리와 줄기에서 보다 낮았다.