원문정보
Comparison of Mycophenolic Acid Production by P. brevicompactum Mutants Induced through UV and NTG Treatments
초록
영어
Recently, importance of immunosuppression is increasing as internal organ transplant becomes more prevalent with development of medical technology. Mycophenolic acid (MPA) is a selective inhibitor of guanine synthesis and it therefore has antibacterial, antiviral, antitumor and selective immunosuppressive activities. The objective of this study was to maximize MPA productivity through utilizing the MPA generating strain of Penicillium brevicompactum ATCC 16024, by inducing UV mutation and NTG mutation. The highest MPA obtained was 1.146 g/L, 2.051 g/L, and 1.390 g/L from P. brevicompactum UB-3, UB-9, UC-4 respectively mutants derived from UV treatment. P. brevicompactum NC-3 and NA-9 induced from NTG treatment yielded. 575 g/L, 2.238 g/L of MPA production respectively. Mutants capable of the highest observed production of MPA were P. brevicompactum UB-9 and P. brevicompactum NC-3 obtained using the UV and NTG treatments respectively.
목차
서론
재료 및 방법
사용균주 및 배양방법
Mycophenolic acid (MPA) 분석
HPLC를 이용한 MPA의 정량
P. brevicompactum 돌연변이
N-methyl-N-nitro-N-nitroguanidine 돌연변이 (NTGmutation)
결과 및 고찰
생물학적 정량을 위한 대상균주 선정
MPA의 추출 및 정량분석
자외선 돌연변이 (UV mutation)
N-methyl-N'-nitro-N-nitrosoguanidine 돌연변이 (NTGmutation)
결론