earticle

영어

In this work, the amounts of Flavone C-glycosides homoorientin, orientin extracted from Black bamboo by various ultrasonic waves frequency (35, 72, 170 KHz, 300 Watt ±1) time (15, 30, 60 min) and temperature (25℃) were compared using 50% aqueous ethanol solution. And describesanalysis of the antioxidant potential of Black bamboo using an high-performance liquid
chromatography (HPLC) on-line ABTS+antioxidant screening method. In conjunction with the analysis of their 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) radical scavenging ability, The optimum operating conditions were experimentally determined to analyze the Flavone C-glycosides homoorientin, orientin in the pretreatment ultrasonic extracts. From the results, the effect on extraction yield of variations in frequency, solvent composition and extraction time was investigated. The highest yield of Black bamboo was obtained by ultrasonic waves with a frequency of 72 KHz and an extraction time of 60 min, The extraction frequency 35 KHz (25℃), time 30 min was selected as an optimal antioxidant activity condition.

한국어

오죽으로부터 HPLC On-line ABTS+ screening기법을 사용하여 Flavone C-glycosides의 특성중의 하나인 항산화활성을 빠르게 분석하였으며, 오죽으로부터 homoorientin와 orientin의 추출을 다양한 초음파 주파수와 시간의 추출방법을 적용하였다. 전 처리한 추출액에 포함된 오죽의 Flavone C-glycosides을 분석하고 최적의 추출조건을 실험적으로 모색하였다. 실험결과에 의하면 전체 수율에서는 Frequency 72 KHz, 60분에서 3.37 mg으로 추출 효율이 높았고, homoorientin와 orientin의 항산화활
성은 Frequency 35 KHz, 30분으로 추출한 시료가 73.8%와 14.8%로 가장 우수 하였다.