원문정보
Effect of Shear Stress on Bovine Aortic Smooth Muscle Cell Growth
초록
영어
Bovine aortic smooth muscle cells cultured on the slide glass were exposed to sheared flow up to 120 hours in flow chamber to see the effect of shear stress on cell growth in wall shear stresses of 0 to 26dyn/cm2From lactate dehydrogenase concentration measurement of the circulating medium, it was shown that sheared flow in the shear stress range did not remove additional smooth muscle cells from the slide glass compared with cells in stationary condition. According to smooth muscle cell counting percm2 of the surface, smooth muscle cells grew fastest in the stationary condition. As the wall shear stress increased, the growth of cells became slower. When the wall shear stress increased over 17dyn/cm2, cell growth was not observed throughout the experiment
한국어
평활근 세포에 대한 shear stress의 영향을 알아보기 위해 슬라이드 글라스위에 배양된 牛 대통맥의 평활근 세포를 120시간 동안 0~26 dyn/cm2기 다른 일정한 shear stress의 유체에 노출하였다. 실험 장치를 순환하는 배지의 lactate dehydrogena ase의 농도측정 결과, 본 연구에서 적용된 shear stress 범위에서는 유체의 흐름으로 인하여 세포가 슬라이드 글라스 표면으로부터 이탈되는 현상은 없었다. 표면 cm2존재하는 세포의 수를 측정한 결과, 평활근 세포는 정체배양시 가장 빨리 성장하였다. 표변에서의 shear stress가 증가할수록 i영활근 세포의 성장은 늦었으며, shear stress가 17 dyn/cm2에서는 세포의 성장이 관찰되지 않았다.
목차
INTRODUCTION
MATERIALS AND METHODS
Smooth Muscle Cell Culture
Flow System
Smooth Muscle Cell Counting
Lactate Dehydrogenase Concentration Measurement
RESULTS AND DISCUSSION
Possibility of Smooth Muscle Cell Washout from the Surface due to the Sheared Flow
Effect of Shear Stress on the Smooth Muscle Cell Growth
요약
ACKNOWLEDGEMENT
REFERENCES