earticle

영어

Mineral salts in medium usually profoundly influence microorganism growth and protein synthesis. In order to produce microbial transglutaminase (MTG) with a high yield from Streptoverticillium mobaraense, we screened the minerals NaCl, CaCl2, CoCl2, FeSO4, ZnSO4, MnSO4 and CuSO4 for MTG fermentation. The results indicated that appropriate FeSO4
concentrations could significantly promote cell growth and stimulate the production of MTG. With 15 mg/L of FeSO4 added to medium, 58% improvements were noted in MTG productivity (2.24 U/mL). NaCl, CaCl2, and CoCl2 enhanced MTG productivity by less than 15%, and the optimal concentrations were determined as 1 g/L, 2 g/L, and 30 mg/L respectively.
Furthermore, it was determined that 7.5 mg/L of ZnSO4 in medium could augment MTG productivity by 20% and induce the stationary phase for MTG production to a period 24 hr earlier. This basic and novel discovery should result in the development of a good complement to the previously defined culture media for MTG fermentation.