원문정보
회분식과 연속식 2단계 생물반응기에서 AcNPV의 곤충세포에의 감염시 배지 첨가물을 이용한 재조합 B-Galactosidase 생산의 증진
초록
영어
The medium additives such as CaCl2, glucose, fructose, glutamine, glutamate and lipids were examined to enhance recombinant B-galactosldase(B-gal) production in batch and continuous two-stage bioreactor systems. The presence of each medium additive such as CaCl2, fructose, glutamate, cholesterol and tocopherol at AcNPV infection of Sf 21 cells had an effect on improved B-gal production. The recombinant B-gal production using the infection media supplemented with a mixture of 30mM , 2.2mM fructose, 4.1mM glutamate and 0.34mM cholesterol was increased by about 40%.
한국어
CaCI2, glucose, frutose, glutamine, glutamate 그리고 Ii pids와 같은 배지 첨가물들이 회분식 그리고 연속식 2단계 생물반응기 시스템에셔 재조합 B-galactosidase (B-gal) 생산을 증진시키는가를 조사하였다. Sf 21 세포에 AcNPV의 감염시 , frutose, glutamate, cholesterol 및 tocoph eral과 같은 배지 첨가물을 첨가하였을 때 B-gal 생산이 증진되었다. 30mM , 2.2mM frutose, 4.lmM glutamine, 그리고 O.34mM cholesterol이 보강된 감염 배 지를 이용한 재조합-gal 생산은 약 40% 의 증가를 보였다.
목차
INTRODUCTION
MATERIALS AND METHODS
Cell Line, Virus and Medium
Batch Culture Conditions
Continuous Two-stage Bioreactor Culture Coditions
Analytical Methods
RESULTS AND DISCUSSION
Batch Culture
Continucus Two-Stage Bioreactor Culture
요약
ACKNOWLEDGEMENT
REFERENCES