원문정보
Lipids Supply for Hybridoma Culture
피인용수 : 0건 (자료제공 : 네이버학술정보)
초록
영어
BSA/acids component in serum free medium (SFM) developed for the culture of hybridoma cell line, KA112, was replaced by acids/Pluronic F-68 emulsion. Protein content of SFM was minimized, and increased maximum cell density was obtained in serum-free lipids supplemented medium (SFLSM). Cell growth promotion effect of the emulsion was not affected by filtration with 0.2um filter.
한국어
기존의 무혈청 배지에 첨가하던 BSA/acid를 acid/Pluronic F-68 emulsion으로 대치하여도 세포성장에는 전혀 지장이 없었다. BSA를 제외함으로써 무혈청 배지에 첨가하는 단백질 함량을 최소하할 수 있었고, 이로 인한 세포농도 감소는 없었다. SFLSM은 0.2micron 필터로 여과 멸균하여도 SFLSM의 세포성장효과는 영향을 받지 않는다.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Cell line
Medium
Cell culture
Chemicals
Preparation of emulsion
RESULTS AND DISCUSSION
Effect of oleic acid amulsion on cell growth
Effect of linoleic acid emulsion on cell growth
Effect of emulsifier change on cell growth
Effect of acids/pluronic F-68 on cell growth
Effect of filtration of SFLSM on cell growh
요약
ABBREVIATION
ACKNOWLEDGEMENT
REFERENCES
INTRODUCTION
MATERIALS AND METHODS
Cell line
Medium
Cell culture
Chemicals
Preparation of emulsion
RESULTS AND DISCUSSION
Effect of oleic acid amulsion on cell growth
Effect of linoleic acid emulsion on cell growth
Effect of emulsifier change on cell growth
Effect of acids/pluronic F-68 on cell growth
Effect of filtration of SFLSM on cell growh
요약
ABBREVIATION
ACKNOWLEDGEMENT
REFERENCES
저자정보
참고문헌
자료제공 : 네이버학술정보