earticle

영어

Up to now, 10% Fetal Bovine Serum(FBS(V/V)) was added to basal medium for the cultivation of hybridoma. For the cultivation of hybridoma cell line, CH07E02, against colon cancer, serum concentration was reduced to 3% FBS without influence on cell growth and maximum cell concentration. By the addition of cell growth promoting substances-insulin (I), pyruvate (P), oxaloacetate(O), Pluronic F-68(P) and 2-mercaptoethanol(2-ME)-to 1% FBS medium, a cell density higher than that with 1% FBS medium alone was achieved. FBS 3% medium was replaced by very cheap 2% Calf Serum (CS) medium without influence on cell growth rate and concentration. Cells grew vigorously in 0.5% CS＋IPOP medium. This composition was used during suspension culture and exhibited good viability and high specific growth rate.

한국어

하이브리도마 배양을 위해 첨가한 기존의 혈 청농도는 10% FBS였으나, 이 농도는 3%까지 줄여도 세포의 비성장속도와 최고세포농도에 차이가 없었다. 세포성장을 촉진한다고 알려진 물 질들을 1% FBS 배지에 첨가하였을 때 insulin, pyruvate, oxaloacetate, pluronic F-68 등에 의해 세포성장이 촉진되었다. 3% FBS는 2% CS로 대치할 수 있었고, 혈청의 농도는 IPOP의 첨가시 0.5 % CS에서도 active proliferation을 보였다. 최종 조성의 배지가격은 기존의 10 % FBS 배지의 1/10 정도였으며 배지조성을 cell line에 따라 달리 결정함으로써 배지의 가격은 현저히 낮출 수 있음을 알았다.