원문정보
Thalicrtrum rugosum 세포배양에 의한 berberine 생산에 미치는 여러 가지 elicitor의 영향
초록
영어
Effects of various elictitors were investigated to enhance the production of berberine in plant cell suspension cultures of Thalicrtrum rugosum. Treatments of yeast elicitor, 15 different types of abiotic elicitors, 16 kinds of fungal elicitors from three species of fungi were performed. Cell growth and berberine production were examined and compared for both normal and elicitor treated cultures. No distinguished increases in berberine yield by the addition of elicitors could be attained.
한국어
Thalicrtrum rugosum 식물세포 현탁배양에 의한 berberine 생산에 미치는 여러 가지 종류의 elicitor들의 영향에 관해 연구 하였다. 효모 유래의 elicitor, 15가지의 서로 다른 종류의 무생물 종류 elicitor, 3가지 곰팡이로부터 얻은 elicitor 들을 처리하여 세포의 생장 및 berberine 생산에 미치는 영향을 비교 분석해 본 결과, 이들 elicitor들은 T. rugosum에 의한 berberine 생산을 크게 증대시키지 못함을 확인하였다
목차
INTRODUCTION
MATERIALS AND METHODS
Isolation of Yeast Elicitors
Preparation of abiotic elicitors
Isolation of elicitors from fungal cultures
Determination of carbohydrate concentration in various biotic elicitors
Alkaloid analysis
RESULTS AND DISCUSSION
Time course responses after addition of yeast elicitor
Examination of abiotic elicitors
Effects of fungal elicitors
ACKNOWLEDGEMENT
요약
REFERENCES