원문정보
Effect of Fungal Elicitor, Pluronic F-68 and Methylcellulose on Suspension Culture of Mentha piperita Cells
초록
영어
The effect of fungal elicitor, Pluronic F-68 and methylcellulose on suspension culture of M piperita cells was investigated in shake flasks. About a two-fold increase in oil production was observed in response to the treatment of the fungal elicitor prepared from Rhodotorula rubra. Low concentration of Pluronic F-68 or methylcellulose enhanced Peppermint cell growth at 100 rpm of agitation.
한국어
Shake flask를 사용하여 M. piperita 세포의 현탁 배양에서의 fungal elicitor, pluronic F-B8, methylcellulose의 영향에 대하여 연구하였다. 그 결과 Rhodotorula rubra라는 균주에서 추출한 fungalelicitor를 처리하여 약 2배 정도 박하정유 생산의 증가를 관찰하였고 100 rpm의 교반속도에서 낮은 농도의 Pluronic F-68, methylcellulose 첨가에 의해 박하세포의 성장이 증진되었다.
목차
INTRODUCTION
MATERIALS AND METHODS
Cell Line and Seed Culture Preparation
Suspension Culture Operation
Fungal Elicitor Preparation
Biomass, Sugar and Essential Oil Analyses
RESULTS AND DISCUSSION
Effect of Fungal Elicitor on Peppermint Oil Production
Effect of Pluronic F-68 abd Methylcellulose on Peppermint Cell Growth
요약
ACKNOWLEDGEMENT
REFERENCES