원문정보
Studies on the Effect of Polyamine on Chlorophyll Contents and Chloroplast Peroxidase Activities in Rice Leaf Segments
초록
영어
The effect of polyamine on chlorophyll amount, chloroplast peroxidase and chloroplast thylakoid protein in rice leaf segments which were grown for 10 days(16 hrs, light : 8 hrs, dark) in a hormone-free MS medium containing polyamine was studied. Polyamine treatment increased the chlorophyll contents compared with the control in rice leaf segments. Especially spermine was most effective. Also, in rice leaf segments treated with polyamine chloroplast peroxidase activity was higher than in the control. The treatment with lmM of spermidine increased the enzyme activity by 100%. In polyamine treatment and control two major polypeptide bands corresponding to 56 and 25Kd molecular weight were clearly resolved with other minor bands by SDS-PAGE in the insoluble protein fraction. However, in these bands (56, 25Kd), the total area of protein in treating with polyamine were higher than that of the control. These results suggest that polyamine was an important factor in the chloroplast development of rice seedlings.
한국어
호르몬이 결여되고 polyamine이 농도별로 처리된 M MS배지에서 10일 동안 발아생장(light, 16hrs: d dark, 8hrs) 시켜서 얻은 벼 잎 절편에서 엽록소 함 량은 polyamine 처리구가 대조구에 비해 높았으며, s spermine(O.OlmM, O.lmM, ImM) 이 가장 효과적이였다. 한편 chloroplast peroxidase의 활성은 polyamine 처리구가 대조구에 비해 전반적으로 높았으며, 특히 1mM spermidine 처리구는 약 100% 정도 활성을 증가시켰다. SDS-PAGE에 의해 chloroplast thylakoid membrane protein의 band를 조사한 결과 polyamine 처리구와 대조구에서 56, 2 25Kd의 major band를 얻었으며 이 band들의 total a area는 polyamine 처리구가 대조구 보다 더 높았다. 이러한 결과들은 벼 유식물에서 엽록체 발달에 p polyamine이 중요한 인자로 작용한 것으로 사료된다
목차
서론
재료 및 방법
실험재료
엽록소 함량측정
엽록체 순수분리
Thylakiod membrane 추출
Chloroplast peroxidase 활성 측정
Tylakoid membrane 단백질의 전기영동적 분석
결과 및 고찰
엽록소 함량의 변화
Chloroplast peroxidase 활성의 변화
Thylakoid membrane protein 조성의 변화
요약
감사
참고문헌