원문정보
Stspension Culture of Betalain Producing Cell-Line and Characteristics of Hairy Root of Phytolacca esculenta V. Houtte
초록
영어
Thc effect of light and phylohormonc on the belalaln synthesis was tested using the suspension culture of a rod(bclalaln produulng) cell line from Phytolacca esculenta V. Houtte. Betalain synthesis of rod-cell 1ine was strongly dependent on the irradiation of blue light, but induced by hormone, IAA and/or kinetin, in dark conditions. In a light condition, however, 2 mg/l of IAA increased the betalain content about 30% (per gram fresh weight), whereas more than 0.5mg/l of kinetin remarkably decreased. The hairy root derived from the same plant was also observed for the blue light-dependent pigmentation in the root-tips. When the hairy root grown in dark was transferred to the light condition, the accumulation of betalain was initiated after 12hours. Such pigmentation was completely inhabited by addition of a protein synthesis inhibitor.
목차
서론
재료 및 방법
장록 캘러스의 세포주 선발
적색세포주의 최적배지 및 호르몬 효과
장록 모상근 유기 및 특성
광조건 및 betalain함량 측정
결과 및 고찰
캘러스의 색소합성에 대한 광의 효과
Betalain합성에 대한 호르몬 효과
장록의 모상근과 특성
요약
감사
참고문헌