원문정보
Studies on the Induction of Transformation in Cereal Plants. III. Cultures and Regeneration of Rice Protoplasts Transferred Foreign Genes
초록
영어
Transformed rice plantlet were recovered from protoplasts by electroporation with the plasmld pB 1121, which contain the plant expressible NPT-II and GUS genes. Embryonic cell suspension culture was established with embryonic callus induced from mature seeds of rice (Oryza sativa L. cv. Dong-jin) on the MS medium supplemented with 2.0 mg/l 2,4-D, 0.5 mg/l kinetin, 3% sucrose. Protoplasts isolated from embryonic cell suspensions were electroplated and then poterltialty-transformed tissues were selected by growth on the medium containing 200 mg/l kanamycin sulfate. When subjected to GUS assay, they stained blue, indicating the expression of the inserted GUS genes. Plantlets were regenerated from electroplated protoplasts on the hormone free MS medium. Transferred foreign genes in the plants were confirmed by southern hybridization. These results support use of electroporation for transformation of these important cereal plants.
목차
서론
재료 및 방법
배 발생 캘러스 유도 및 배양
배 발생세포의 현탁배양
사용균주 및 Plasmid DNA
Plasmid DNA의 분리
원형질체 분리 및 electroporation
원형질체 배양 및 재분화
형질전환 확인
결과 및 고찰
적요
감사
참고문헌