원문정보
Production of D-sorbitol and L-sorbose from Jerusalem artichoke by Zymomonas mobilis and Gluconobacter sMboxpydans
초록
영어
The use of Jerusalem artichoke containing B-1, 2-fructose oligomer for the production of D-sorbitol and L-sorbose has been studied. The employment of inulinase(0.398%, v/v) for the hydrolysis of 40% (v/w) Jerusalem artichoke juice resulted in 36.7g/1 of glucose and 85.3g/1 of fructose at 50℃. These sugars were utilized as substrates for D-sorbitol and L-sorbose production. Coimmobilization of inulinase and permeabilized cells of Zymomonas mobilis in the mixture of chitin (5%, w/e) and x-carrageenan(4%, w/v) resulted in the production of 30.2g/1 of D-sorbitol by using inulin as a substrate. The process of L-sorbose production from D-sorbitol by Gluconobacter suboxydans was optimized with respect to the substrate concentration, level of dissolved oxygen and glucosic and concentration. Gluconlc acid produced by Zymomonas mobilis from glucose was found to inhibit Gluconobacter suboxtans in conversion of D-sorbitol to L-sorbose. In view of removing such inhibitory effect by gluconic acid, mutants were selected by the NTG (N-methyl-N'-N'-nitro-N-nitrosoguanidlne) treated method. Mutants selected by NTG mutagenesis showed no inhibitory effects of gluconic acrid against L-sorbone production when its concentration increased up to 100g/1. A mutant produced 40.1g/l of L-sorbose in the medium containing 100g/l D-sorbitol and 100g/l-gluconic acid. This result is consider able when compared with L-sorbose concentration (21.7g/1) obtained from the fermentation with wild type strain of Gluconobacter suboxnians.
목차
서론
재료 및 방법
균주 및 배지조성
세포의 투과성 증진
돼지감자즙의 제조 및 가수분해
효소와 세포의 동시고정화
UV조사에 의한 돌연변이 선발
NTG처리에 의한 돌연변이 선발
분석 방법
결과 및 고찰
돼지감자즙의 가수분해
D-Sorbitol 생산조건의 최적화
Zymomoeas mobils 세포와 inulinase의 동시 고정화
L-sorbose의 생성에 미치는 용존산소의 영향
L-sorbose생성에 미치는 D-sorbitol 및 gluconic acid의 영향
Gluconic acid 내성 돌연변이주 선발
요약
참고문헌